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The mass spectrometer was operated in electron impact ionization mode with an electron energy of 70 eV and a trap current of 100 μA; ion source temperature was 200°C. After a 1 minute solvent delay, a mass spectrum was acquired every second during the run using a scan range of m/z 50-50, a scan rate of 0.8 seconds/decade, and an interscan time of 0.2 seconds. Electrospray ionization mass spectrometry (ESI) was performed using a model 5555-22 syringe pump (Harvard Apparatus, South Natick, MA, USA) connected to a model TWQ7000 mass spectrometry/mass spectrometry (MS/MS) instrument (Finnigan MAT, San Jose, CA, USA) via the ESI interface. A sample of approximately 10 μg in 250 μL methanol:water (1:1) was infused at a rate of 10 μL/minute. Nitrogen was used as a sheath gas at a head pressure of 70 psi and as an auxiliary gas at a flow rate of 500 mL/minute. A negative ion ESI was recorded by averaging several Q1 scans (mass range = m/z 80-700, scan rate of 3 seconds/scan) during sample infusion..
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